My PhD focuses on applying high-resolution sequencing technologies to tumors like neuroblastoma and melanoma to understand their complex ecosystems. Tumors comprise diverse cell types and are influenced by the tumor microenvironment (TME), which impacts treatment resistance and metastasis. While single-cell RNA sequencing identifies cell populations, it lacks spatial context, which is addressed by high-resolution spatial transcriptomics. This approach allows us to study cellular interactions and malignant states within the TME, potentially leading to new biomarkers and therapeutic strategies. By investigating neuroblastoma and melanoma samples, our research aims to enhance understanding of the TME and improve treatment outcomes.