| Dra I enzyme |
NEB |
Cat#R0129 |
| Alkaline Phosphatase Calf Intestinal (CIAP) enzyme |
Promega |
Cat#M1821 |
| Exonuclease I enzyme |
NEB |
Cat#M0293 |
| NaOH solution for molecular biology 10 M in H2O |
Sigma |
Cat#72068 |
| UltraPure™1M Tris-HCl pH7.5 |
Invitrogen™ |
Cat#15567027 |
| Tissue-Tek OCT |
Sakura Finetek |
Cat#4583 |
| Methanol (min. 99.8%) |
Th. Geyer |
Cat#1437 |
| 2-propanol (min 99.9%) |
Th. Geyer |
Cat#1197 |
| Mayer’s Haematoxylin |
Agilent Dako |
Cat#S3309 |
| Bluing buffer |
Agilent Dako |
Cat#CS702 |
| Eosin Y, aqueous |
Sigma |
Cat#HT110216 |
| Pepsin from porcine gastric mucosa |
Sigma |
Cat#P7000 |
| 20x SSC |
Sigma |
Cat#S6639-1L |
| Hydrochloric Acid (HCl) 10N |
AppliChem |
Cat#187051 |
| BSA Molecular Biology Grade (conc. 20 mg/ml) |
NEB |
Cat#B9000S |
| dNTP SET 100mM 4X1mL |
Life Technologies |
Cat#R0182 |
| SuperScript IV Reverse Transcriptase |
Life Technologies |
Cat#18090010 |
| RiboLock RNase Inhibitor |
Thermo Scientific |
Cat#EO0381 |
| Tris-HCl Buffer pH 8.0, 1M |
Life Technologies |
Cat#AM9855G |
| Sodium chloride NaCl (5M), RNase-free |
Invitrogen |
Cat#AM9760G |
| Roti®-Stock 20 % SDS ready-to-use, sterile filtered |
Roth |
Cat#1057.1 |
| UltraPure 0.5M EDTA, pH 8.0 |
Life Technologies |
Cat#15575020 |
| Proteinase K (800 mU/μL) |
NEB |
Cat#P8107S |
| DNA Polymerase Large Fragment exo- Klenow Fragment (3'-5' exo-) |
NEB |
Cat#M0212 |
| Ampure XP beads |
Beckman coulter |
Cat#A63881 |
| Kapa HiFi Hotstart Readymix KK2612 |
Roche |
Cat#7958960001 |
| 1.5% Agarose gel, PippinHT (300-1500 bp) |
Biozym |
HTC1510 |
| Qubit dsDNA HS Assay Kit |
Invitrogen |
Cat#Q32854 |
| High sensitivity DNA kit |
Agilent |
Cat#5067-4626 |
| HS RNA tapestation |
Agilent |
5067-5579/5580/5581 |
| Blue S'Green qPCR mix |
Biozym |
Cat#331416 |
| KAPA LQ Primer + Mastermix (Illumina/ LC480) |
Roche |
Cat#7960573001 |
| KAPA Library Quantification DNA Standards (Illumina) |
Roche |
Cat#7960387001 |
| NovaSeq 6000 S4 reagent kit v1.5 (35 cycles) |
Illumina |
Cat#20044417 |